Beauvericin detoxification compositions and methods

The present invention provides a bacterial microorganism having the ability to degrade or neutralize beauvericin or derivatives or analogs of beauvericin. The present invention further provides a method for detoxification of plants pre- or post-harvest using microbes having the ability to neutralize or purify beauvericin or derivatives or analogs of beauvericin.


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Fungal diseases are common problems in crop agriculture. Many strides have been made against plant diseases as exemplified by the use of hybrid plants, pesticides and unproved agricultural practices. However, as any grower or home gardener canattest, the problems of fungal plant disease continue to cause problems in plant cultivation. Thus, there’s a continuing need for new methods and materials for solving the problems caused by fungal infections of plants. These problems can be metthrough a variety of approaches. For example, the infectious organisms may be controlled via the use of brokers that are selectively biocidal for its pathogens. Another procedure is interference with the mechanism by which the pathogen invades the hostcrop plant. Still another procedure, in the case of germs that cause crop losses, is interference with the mechanism by which the pathogen causes injury to the host crop plant. Another method, in the case of germs that produce toxins that areundesirable to mammals or other animals that feed on the crop plants, is interference with poison production, storage, or action.


Inside the Fusarium sp.

Are several important elements of corn and other cereals in various countries. In corn, Fusarium is known to cause root, stem and ear rot that leads to severe crop loss. The etiology of Fusarium ear mould ispoorly understood, although physical harm to the ear and also certain environmental conditions can contribute to its occurrence(Nelson PE (1992)”Taxonomy and Biology of Fusarium moniliforme.” Mycopathologia 117: 29-36). Fusarium might be isolated from mostfield grown maize, when no visible mold is present. The relationship between seedling infection and the stalk and ear ailments caused by Fusarium isn’t clear. Genetic resistance to observable kernel mould was identified. (Gendloff E, Rossman E, CasaleW, Isleib T, Hart P, 1986,”Components of resistance to Fusarium ear rot in field corn” Phytopathology 76: 684-688; Holley RN, Hamilton PB, Goodman MM, 1989,”Evaluation of tropical maize germplasm for resistance to kernel colonization by Fusariummoniliforme.” Plant Dis 73: 578-580). The mycotoxins made by the Fusarium species that infect plants can accumulate in infected plants or in stored fats, demonstrating serious health consequences for livestock, humans, and other customers of beef orother foods of such livestock. Fusarium infection was associated with chronic or acute mycotoxicoses in the farm animals and man (Botallico, et al.). A significant mycotoxin that’s been proven to be made by certain Fusarium sp. Andhas been identified in Fusarium infected crops is beauvericin.

Beauvericin is a fungal toxin produced by various Fusarium species, as well as the fungus Beauveria bassiana. Beauvericin is a cyclic peptide, with toxic effects on insects as well as both human and murine cell lines. The action ofbeauvericin is a result of the ionophoric properties of this compound. Beauvericin is capable of forming complexes with alkali metal cations and affects ion transport across cell membranes. Additionally, beauvericin has been reported to be one of the mostpowerful inhibitors of cholesterol acetyltransferase. Beauvericin has also been shown to induce a type of cell death very much like apoptosis. Circumstantial evidence further suggests that beauvericin functions in concert with other Fusarium toxins tocause further toxic effects (1).

Beauvericin was reported to be seen at significant levels in corn in Italy, Peru, and Poland (1,2,3). It is likely that beauvericin will also be found in different regions as more studies have been finished. Fusarium species are found in virtuallyall moldy as well as healthy corn. Food security is a significant problem for grain growers. The European Commonwealth is considering imposing limitations on several mycotoxin levels of grain.

There’s a need in the art for novel methods by which beauvericin might be removed from a plant or chosen grain. It is deemed important by those skilled in the art to continue to create inventions so as to protect the finalconsumer of a plant or chosen grain. The current invention provides that the reagents and methodologies necessary to ameliorate crops and harvested grains out of beauvericin.

IP reviewed by Plant-Grow agriculture technology news