Card for use in an automated microbial detection system
Improved cards for use in an automated machine to discover the presence of specific microbes and hence identify themenumerate themand/or ascertain their susceptibility to antibiotics. Each card includes a plate having conical detection wells each with a chosen medium therein, at least filling port using a septum that’s retained by a collar formed there, along with filler passages of specific length and configuration linking the port to the wells. Discontinuities are provided in the filler passages to prevent undesirable fluid flow and to decrease migration of these microbes once the card is currently in use. Overflow chambers of a specific configuration can also be provided adjoining the wells to entrap bubbles.
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This invention relates in general to developments to cards containing specific media utilized to ascertain the identity, quantity and antibiotic susceptibility of microorganisms.
There are currently existing procedures and apparatus for determining the presence, identity and quantity of microorganisms and their susceptibility to antibiotics that involve putting the germs into micro-quantities of civilization mediasome of which can sustain specific microorganisms. The press are organized so the optical characteristics of every changes in a predetermined fashion once the medium is keeping the desired form or types of microorganisms.
A convenient method to execute these processes is through the usage of a card or tape constructed with a rigid body in the form of a plastic plate, including viewing chambers or molds that pass through the plate. Every one of those wells isconnected to a predetermined liquid ore supply port by way of channels cut in one side of the plate. Each port comprises a septum, constructed from appropriate sealing compounds, which permits passage of a hollow needle when a liquid ore is beingintroduced into the vent.
The card is built by first forming the plastic plate, including the septum and covering one side of this plate with adhesive tape. Chosen selective press in liquid form are subsequently placed in the proper wells and freeze dried. The oppositeside of this plate is then covered with another layer of adhesive tape to shape, in consequence, a sealed container with a minumum of one supply interface, a plurality of wells and a piping system therebetween. Unfortunately, before the liquid websites are dried, theytend to flow down the connecting grooves by way of capillary or alternative actions and mix with the media of different chambers. This is undesirable since it can cause erroneous results when the card is used.
To introduce a specimen in the viewing wells, a vacuum is drawn via a hypodermic needle inserted through the septum in the filling port. Then atmospheric pressure is used to induce the liquid specimen into your card. This causesboth the wells along with the adjoining branches to be full of liquid containing microorganisms. When no means are supplied to prevent it, the microorganisms, which can be sustained by the press in the seeing wells, tend to migrate with all the reconstituted mediathrough the branches into other colonies. This is undesirable since it can also cause incorrect results when the card is read. There has also been a tendancy for its sealing compound which creates the septum to become dislodged through the card fillingoperation. This destroys the usefulness of the card.
Another problem results in the fact that a number of the metabolic processes of the microbes produce gases which form bubbles at the viewing wells. Overflow chambers are typically provided adjacent the wells to supply for a few bubbles such asthose that result from the filling process, however, the metabolic bubbles tend to form in random fashion in the colonies so that they interfere with the optical reading .
IP reviewed by Plant-Grow agriculture technology news