Delivery of disease control in aquaculture and agriculture using microbes containing bioactive proteins

A microbial biomass, made from algae, algae, fungi, yeast, or mixtures thereof, provides a feed for animals increased either in agriculture or aquaculture. A feed additive, and a therapeutic composition may also be created from a microbial biomass of algae, bacteria, parasites, yeast, or mixtures thereof. The nourish, feed additive, and therapeutic composition may comprise one or more proteins, peptides, antibodies, antibody fragments, or some combination thereof, wherein said proteins, peptides, antibodies, antibody fragments, or some mix thereof are non-native into the microbes of the biomass. The biomass may have therapeutic, bioactive, nutrient, or immunogenic properties.


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1. Field of the Invention

This invention is directed to animal feeds used in aquaculture or in agriculture, with microbial cells as components. These microbial cells comprise exogenous peptides, proteins, and/or antibodies, which can convey immunity or resistance topathogens (like viral or bacterial), or otherwise improve the health and performance of the species that have them. The cells can be algae, bacteria, fungi, or yeast. The exogenous peptides, proteins, and/or antibodies can be expressedinside the cells by direct genetic modification of the microbe or by trapping the microbe with a virus that has been modified to express the protein of interest. The invention is also directed to animal feed supplements and therapeutics withmicrobial cells as components.

2. Related Art

Plant products have been produced using specific genetic modification to express electrons or proteins of therapeutic value. The team in the Boyce Thompson Institute at Cornell has cloned a viral coat protein to bananas capabledelivering a oral vaccine when ingested by people, but this notion has not been extended to germs.

There are lots of plant biotech companies, such as Meristem, Large Scale Biology, and Prodigene, which are currently expressing certain human therapeutic proteins, including antibodies, in plants. Large Scale Biology is expressing proteins in tobaccoplants employing a tobacco mosaic virus as a vector to create the protein of interest. The protein is then isolated and purified from the plant material and used for human therapeutic functions. In this way, the plant genome itself is actually notmodified, but rather the genome of the infecting virus carries the gene of interest.

Recombinant germs, such as bacteria, yeast, and other parasites, have been utilized to produce human therapeutic proteins. But, such recombinant microbes have yet to be utilized in agriculture or agriculture, whereas the cultivated animal ingeststhe whole organism. Rather, up to now, the recombinant organism has been used as a factory from which the therapeutic protein is isolated and purified before use.

Certain plant compounds have been produced that contain electrons or proteins of curative value. They’ve been made by means of plant using a virus that expresses the protein of interest. Large Scale Biology includes a series ofpatents shielding this technology, but its purpose is to create purified proteins for pharmaceutical purposes, which demands an extensive purification procedure following harvesting of this plant substance. These patents don’t involve using thecrude plant material as a source of both nutrition and disease management, except under the odd condition that the pharmaceutical product is extracted from the fruit of this plant.

Certain recombinant proteins have been produced in insect cells with a bug virus expression system (baculovirus). These proteins are also produced in intact insect larvae following disease with modified baculoviruses. In both cases, theinsect cells or larvae are used as factories to produce the protein of interest, and the recombinant protein is then purified for pharmaceutical purposes. Insect cells or larvae infected with baculovirus are especially beneficial in the expression ofcertain human therapeutic proteins because the post-translational modifications of those therapeutic proteins are just like the post-translational modifications imparted upon expression in human cells.

A baculovirus expression system is also an efficient way of expressing proteins in insect cell culture. Baculovirus is at the family Baculoviridae, a diverse group of large double stranded DNA viruses which infect arthropods, including insects,arachnids, and crustaceans. Baculoviruses are species-specific and do not infect vertebrates, nor can they propagate in mammalian cells in culture.

The Sindbis arbovirus can be used to provide high levels of gene expression in vivo in non-host arthropod species without causing cytopathic effects in infected cells or impairing the evolution of the organism. A replication competent Sindbisvirus comprising the coding region of green fluorescent protein (GFP) induced productive ailments when injected into insect larvae and pupae (Lewis, et al., 1999). Therefore, virus-mediated ectopic gene expression was accomplished in arthropods, aphylum that comprises the courses Crustacea and Insecta.

Antibiotic doping is utilized regularly in the aquaculture setting. Typically, the pure or semipure antibiotics have been added directly to the water column. However, primitive fermentation broths, or primitive preparations such as cells, haven’t been usedfor any kind of therapeutic delivery method.

Generation of amino acids, such as lysine, usually involves a genetically modified microorganism, which overproduces the amino acid of attention and divides it to the fermentation medium. The wastestream from such a fermentation wouldinclude biomass comprising the amino acid, and also this wastestream product could be used as a crude shipping form of the small molecule lipoic amino acid.

Microalgae (single celled algae or phytoplankton) represent the biggest, but most badly understood, kingdom of microorganisms in the world. As plants would be to terrestrial animals, microalgae signify the organic nutritional foundation and primarysource of all the phytonutrients in the aquatic food chain. Since the principal producers in the aquatic food chain, microalgae will be the source of numerous phytonutrients, including docosahexaenoic acid (DHA) and arachidonic acid (ARA). Microalgae additionally representa vast genetic source, comprising in excess of 80,000 distinct species.

Yeast, filamentous fungi, and bacteria are also in the immediate food chain of fish, crustaceans, and mollusks. However, just a very few of those microbes, less than 10 species, have been manipulated for aquaculture feeds. These few specieshave been used primarily for historic reasons and ease of cultivation. They have never been selected on the basis of any scientific evidence of excellence as supplements or therapeutic nutritional supplements.

IP reviewed by Plant-Grow agriculture technology news